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cell mitochondrial complex iii activity assay 19 kit  (Elabscience Biotechnology)


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    Elabscience Biotechnology cell mitochondrial complex iii activity assay 19 kit
    Cell Mitochondrial Complex Iii Activity Assay 19 Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mitochondrial+complex+iii+activity+assay+kit/pm41654147-130-26-35?v=Elabscience+Biotechnology
    Average 94 stars, based on 8 article reviews
    cell mitochondrial complex iii activity assay 19 kit - by Bioz Stars, 2026-07
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    Astragaloside IV improves SCM myocardial inflammatory injury via DUSP1-Prohibitin 2(PHB2). (A) Immunofluorescence staining showed the expression of Tnt and Gr-1 in cardiac tissue. The images showed the expression changes in the control group (Ctrl), LPS-treated group, and after treatment with different concentrations of AS (AS-L, AS-M, and AS-H). (B) The levels of Gr-1 in mice were detected. Scale bar, 150 μm. (C–E)Protein expression and transcriptional level detection of DUSP1/PHB2 (F–H) Echocardiography was used to analyze the cardiac function of mice. The parameters analyzed included left ventricular ejection fraction (LVEF%), FS%, and LVDd. (I-K) The levels of serum myocardial injury markers, including creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), and troponin (TnT), were detected. (L-N) Analysis of inflammatory factor levels, detecting changes in MMP-9, interleukin-10 (IL-10), and interleukin-17 (IL-17).(O-Q) Transcription level detection of <t>mitochondrial</t> pathway necroptosis mediated genes (Caspase-3/-9/-12). Statistical conditions: Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of <t>three</t> replicates in each animal. *p < 0.05.
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    mitochondrial complex iii activity assay kit  (Elabscience Biotechnology)
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    Elabscience Biotechnology mitochondrial complex iii activity assay kit
    Astragaloside IV improves SCM myocardial inflammatory injury via DUSP1-Prohibitin 2(PHB2). (A) Immunofluorescence staining showed the expression of Tnt and Gr-1 in cardiac tissue. The images showed the expression changes in the control group (Ctrl), LPS-treated group, and after treatment with different concentrations of AS (AS-L, AS-M, and AS-H). (B) The levels of Gr-1 in mice were detected. Scale bar, 150 μm. (C–E)Protein expression and transcriptional level detection of DUSP1/PHB2 (F–H) Echocardiography was used to analyze the cardiac function of mice. The parameters analyzed included left ventricular ejection fraction (LVEF%), FS%, and LVDd. (I-K) The levels of serum myocardial injury markers, including creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), and troponin (TnT), were detected. (L-N) Analysis of inflammatory factor levels, detecting changes in MMP-9, interleukin-10 (IL-10), and interleukin-17 (IL-17).(O-Q) Transcription level detection of <t>mitochondrial</t> pathway necroptosis mediated genes (Caspase-3/-9/-12). Statistical conditions: Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of <t>three</t> replicates in each animal. *p < 0.05.
    Mitochondrial Complex Iii Activity Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Astragaloside IV improves SCM myocardial inflammatory injury via DUSP1-Prohibitin 2(PHB2). (A) Immunofluorescence staining showed the expression of Tnt and Gr-1 in cardiac tissue. The images showed the expression changes in the control group (Ctrl), LPS-treated group, and after treatment with different concentrations of AS (AS-L, AS-M, and AS-H). (B) The levels of Gr-1 in mice were detected. Scale bar, 150 μm. (C–E)Protein expression and transcriptional level detection of DUSP1/PHB2 (F–H) Echocardiography was used to analyze the cardiac function of mice. The parameters analyzed included left ventricular ejection fraction (LVEF%), FS%, and LVDd. (I-K) The levels of serum myocardial injury markers, including creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), and troponin (TnT), were detected. (L-N) Analysis of inflammatory factor levels, detecting changes in MMP-9, interleukin-10 (IL-10), and interleukin-17 (IL-17).(O-Q) Transcription level detection of <t>mitochondrial</t> pathway necroptosis mediated genes (Caspase-3/-9/-12). Statistical conditions: Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of <t>three</t> replicates in each animal. *p < 0.05.
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    Astragaloside IV improves SCM myocardial inflammatory injury via DUSP1-Prohibitin 2(PHB2). (A) Immunofluorescence staining showed the expression of Tnt and Gr-1 in cardiac tissue. The images showed the expression changes in the control group (Ctrl), LPS-treated group, and after treatment with different concentrations of AS (AS-L, AS-M, and AS-H). (B) The levels of Gr-1 in mice were detected. Scale bar, 150 μm. (C–E)Protein expression and transcriptional level detection of DUSP1/PHB2 (F–H) Echocardiography was used to analyze the cardiac function of mice. The parameters analyzed included left ventricular ejection fraction (LVEF%), FS%, and LVDd. (I-K) The levels of serum myocardial injury markers, including creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), and troponin (TnT), were detected. (L-N) Analysis of inflammatory factor levels, detecting changes in MMP-9, interleukin-10 (IL-10), and interleukin-17 (IL-17).(O-Q) Transcription level detection of mitochondrial pathway necroptosis mediated genes (Caspase-3/-9/-12). Statistical conditions: Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Journal: Journal of Advanced Research

    Article Title: Astragaloside IV alleviates septic myocardial injury through DUSP1-Prohibitin 2 mediated mitochondrial quality control and ER-autophagy

    doi: 10.1016/j.jare.2024.10.030

    Figure Lengend Snippet: Astragaloside IV improves SCM myocardial inflammatory injury via DUSP1-Prohibitin 2(PHB2). (A) Immunofluorescence staining showed the expression of Tnt and Gr-1 in cardiac tissue. The images showed the expression changes in the control group (Ctrl), LPS-treated group, and after treatment with different concentrations of AS (AS-L, AS-M, and AS-H). (B) The levels of Gr-1 in mice were detected. Scale bar, 150 μm. (C–E)Protein expression and transcriptional level detection of DUSP1/PHB2 (F–H) Echocardiography was used to analyze the cardiac function of mice. The parameters analyzed included left ventricular ejection fraction (LVEF%), FS%, and LVDd. (I-K) The levels of serum myocardial injury markers, including creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), and troponin (TnT), were detected. (L-N) Analysis of inflammatory factor levels, detecting changes in MMP-9, interleukin-10 (IL-10), and interleukin-17 (IL-17).(O-Q) Transcription level detection of mitochondrial pathway necroptosis mediated genes (Caspase-3/-9/-12). Statistical conditions: Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Article Snippet: NO: Mouse MMP-9(Matrix Metalloproteinase 9) ELISA Kit E-EL-M3052, Elabscience, China);Mouse IL-10(Interleukin 10) ELISA Kit(E-EL-M0046, Elabscience, China);Mouse IL-17A(Interleukin 17A) ELISA Kit(E-EL-M0047, Elabscience, China);MDA(Malondialdehyde) ELISA Kit(E-EL-0060, Elabscience, China);A001-2-1 Superoxide Dismutase (SOD) Typing Test Kit(E-BC-K020-M, Elabscience, China);H545-1-1 Glutathione Peroxidase 4 (gpx4) Kit(E-BC-K096-S, Elabscience, China);Cell Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit(E-BC-K834-M, Elabscience, China);Cell Mitochondrial Complex V (F0F1-ATPase/ATP Synthase) Activity Assay Kit (E-BC-K838-M, Elabscience, China);Cell Mitochondrial Complex III (Coenzyme Q-Cytochrome C Reductase) Activity Assay Kit(E-BC-K836-M, Elabscience, China);.

    Techniques: Immunofluorescence, Staining, Expressing, Control

    Astragaloside IV inhibits pyroptosis after sepsis induced myocardial injury through synergistic of mitophagy and UPRmt. (A) Immunofluorescence staining showed the expression of Tnt and Gr-1 in cardiac tissue (DAPI was used as an internal reference). Scale bar, 150 μm. (B-D) Echocardiography was used to analyze the cardiac function of mice. The parameters analyzed included left ventricular ejection fraction (LVEF%), FS%, and LVDd. (E-G) Serum myocardial injury markers were measured, including creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), and troponin (TnT). (H-J) Analysis of apoptosis-related indicators, including the expression levels of caspase-1, caspase-3, and caspase-9. (K-M) Expression levels of autophagy-related genes, including FUNDC1, ATG5, and ATG12. (N-R) Transcription level of mitochondrial UPR-related gene. (S)Transcription level of PHB2 (T-O) Detection of expression levels of cell pyroptosis and mitochondrial autophagy related proteins. Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Journal: Journal of Advanced Research

    Article Title: Astragaloside IV alleviates septic myocardial injury through DUSP1-Prohibitin 2 mediated mitochondrial quality control and ER-autophagy

    doi: 10.1016/j.jare.2024.10.030

    Figure Lengend Snippet: Astragaloside IV inhibits pyroptosis after sepsis induced myocardial injury through synergistic of mitophagy and UPRmt. (A) Immunofluorescence staining showed the expression of Tnt and Gr-1 in cardiac tissue (DAPI was used as an internal reference). Scale bar, 150 μm. (B-D) Echocardiography was used to analyze the cardiac function of mice. The parameters analyzed included left ventricular ejection fraction (LVEF%), FS%, and LVDd. (E-G) Serum myocardial injury markers were measured, including creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), and troponin (TnT). (H-J) Analysis of apoptosis-related indicators, including the expression levels of caspase-1, caspase-3, and caspase-9. (K-M) Expression levels of autophagy-related genes, including FUNDC1, ATG5, and ATG12. (N-R) Transcription level of mitochondrial UPR-related gene. (S)Transcription level of PHB2 (T-O) Detection of expression levels of cell pyroptosis and mitochondrial autophagy related proteins. Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Article Snippet: NO: Mouse MMP-9(Matrix Metalloproteinase 9) ELISA Kit E-EL-M3052, Elabscience, China);Mouse IL-10(Interleukin 10) ELISA Kit(E-EL-M0046, Elabscience, China);Mouse IL-17A(Interleukin 17A) ELISA Kit(E-EL-M0047, Elabscience, China);MDA(Malondialdehyde) ELISA Kit(E-EL-0060, Elabscience, China);A001-2-1 Superoxide Dismutase (SOD) Typing Test Kit(E-BC-K020-M, Elabscience, China);H545-1-1 Glutathione Peroxidase 4 (gpx4) Kit(E-BC-K096-S, Elabscience, China);Cell Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit(E-BC-K834-M, Elabscience, China);Cell Mitochondrial Complex V (F0F1-ATPase/ATP Synthase) Activity Assay Kit (E-BC-K838-M, Elabscience, China);Cell Mitochondrial Complex III (Coenzyme Q-Cytochrome C Reductase) Activity Assay Kit(E-BC-K836-M, Elabscience, China);.

    Techniques: Immunofluorescence, Staining, Expressing

    Astragaloside IV treatment of LPS-induced mitochondrion dysfunction in cardiomyocytes. The control group (Control) and the high-concentration astragaloside IV (AS-H) treatment group were used as controls. The LPS treatment group, as well as the changes in inflammatory damage in each group after treatment with low, medium, and high concentrations of astragaloside IV (AS-L, AS-M, and AS-H) under certain conditions. (A) Stability testing of myocardial cytoskeletal proteins. Scale bar, 25 μm. (B)CCK8 cell activity detection. (D,E) Analysis of mitochondrial oxidative stress-related indicators. The parameters analyzed included the expression level of ROS and malondialdehyde (MDA) activity. (F,G) Antioxidant enzyme activity analysis. The parameters analyzed included superoxide dismutase (SOD) activity and glutathione peroxidase (GPX) activity. (H-J) Mitochondrial complex activity and ROS analysis Scale bar, 150 μm. The parameters analyzed included the activity changes of complex III (Complex-Ⅲ)/complex V (Complex-Ⅴ) and ROS expression. (K-L) Expression level/transcriptional level of DUSP1.Scale bar, 150 μm. (M)Transcription level of PHB2. (N)CCK8 detection of cell activity (DUSP1 gene modification). (O)Transcription level of PHB2；(P-S)Transcription levels of regulatory genes related to cell pyroptosis；Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Journal: Journal of Advanced Research

    Article Title: Astragaloside IV alleviates septic myocardial injury through DUSP1-Prohibitin 2 mediated mitochondrial quality control and ER-autophagy

    doi: 10.1016/j.jare.2024.10.030

    Figure Lengend Snippet: Astragaloside IV treatment of LPS-induced mitochondrion dysfunction in cardiomyocytes. The control group (Control) and the high-concentration astragaloside IV (AS-H) treatment group were used as controls. The LPS treatment group, as well as the changes in inflammatory damage in each group after treatment with low, medium, and high concentrations of astragaloside IV (AS-L, AS-M, and AS-H) under certain conditions. (A) Stability testing of myocardial cytoskeletal proteins. Scale bar, 25 μm. (B)CCK8 cell activity detection. (D,E) Analysis of mitochondrial oxidative stress-related indicators. The parameters analyzed included the expression level of ROS and malondialdehyde (MDA) activity. (F,G) Antioxidant enzyme activity analysis. The parameters analyzed included superoxide dismutase (SOD) activity and glutathione peroxidase (GPX) activity. (H-J) Mitochondrial complex activity and ROS analysis Scale bar, 150 μm. The parameters analyzed included the activity changes of complex III (Complex-Ⅲ)/complex V (Complex-Ⅴ) and ROS expression. (K-L) Expression level/transcriptional level of DUSP1.Scale bar, 150 μm. (M)Transcription level of PHB2. (N)CCK8 detection of cell activity (DUSP1 gene modification). (O)Transcription level of PHB2；(P-S)Transcription levels of regulatory genes related to cell pyroptosis；Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Article Snippet: NO: Mouse MMP-9(Matrix Metalloproteinase 9) ELISA Kit E-EL-M3052, Elabscience, China);Mouse IL-10(Interleukin 10) ELISA Kit(E-EL-M0046, Elabscience, China);Mouse IL-17A(Interleukin 17A) ELISA Kit(E-EL-M0047, Elabscience, China);MDA(Malondialdehyde) ELISA Kit(E-EL-0060, Elabscience, China);A001-2-1 Superoxide Dismutase (SOD) Typing Test Kit(E-BC-K020-M, Elabscience, China);H545-1-1 Glutathione Peroxidase 4 (gpx4) Kit(E-BC-K096-S, Elabscience, China);Cell Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit(E-BC-K834-M, Elabscience, China);Cell Mitochondrial Complex V (F0F1-ATPase/ATP Synthase) Activity Assay Kit (E-BC-K838-M, Elabscience, China);Cell Mitochondrial Complex III (Coenzyme Q-Cytochrome C Reductase) Activity Assay Kit(E-BC-K836-M, Elabscience, China);.

    Techniques: Control, Concentration Assay, Activity Assay, Expressing, Modification

    Astragaloside IV treatment of LPS-induced MQS dysfunction in cardiomyocytes through PHB2. (A-C) Mitochondrial oxidative stress injury (ROS/SOD/GSH) Scale bar, 150 μm. (D-E) Detection of mitochondrial respiratory chain complexes. (F-J)Detection of mitochondrial energy metabolism levels. Scale bar, 150 μm. (K-N)Mitochondrial membrane potential level detection(JC-1).(O)Endoplasmic reticulum structural integrity testing. Scale bar, 25 μm. (P-R) Detection of transcriptional levels of genes regulating mitochondrial biosynthesis. (S-Y) Mitochondrial dynamics (fusion/fission) related gene transcription levels and mitochondrial structural integrity detection Scale bar, 25 μm.; Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Journal: Journal of Advanced Research

    Article Title: Astragaloside IV alleviates septic myocardial injury through DUSP1-Prohibitin 2 mediated mitochondrial quality control and ER-autophagy

    doi: 10.1016/j.jare.2024.10.030

    Figure Lengend Snippet: Astragaloside IV treatment of LPS-induced MQS dysfunction in cardiomyocytes through PHB2. (A-C) Mitochondrial oxidative stress injury (ROS/SOD/GSH) Scale bar, 150 μm. (D-E) Detection of mitochondrial respiratory chain complexes. (F-J)Detection of mitochondrial energy metabolism levels. Scale bar, 150 μm. (K-N)Mitochondrial membrane potential level detection(JC-1).(O)Endoplasmic reticulum structural integrity testing. Scale bar, 25 μm. (P-R) Detection of transcriptional levels of genes regulating mitochondrial biosynthesis. (S-Y) Mitochondrial dynamics (fusion/fission) related gene transcription levels and mitochondrial structural integrity detection Scale bar, 25 μm.; Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Article Snippet: NO: Mouse MMP-9(Matrix Metalloproteinase 9) ELISA Kit E-EL-M3052, Elabscience, China);Mouse IL-10(Interleukin 10) ELISA Kit(E-EL-M0046, Elabscience, China);Mouse IL-17A(Interleukin 17A) ELISA Kit(E-EL-M0047, Elabscience, China);MDA(Malondialdehyde) ELISA Kit(E-EL-0060, Elabscience, China);A001-2-1 Superoxide Dismutase (SOD) Typing Test Kit(E-BC-K020-M, Elabscience, China);H545-1-1 Glutathione Peroxidase 4 (gpx4) Kit(E-BC-K096-S, Elabscience, China);Cell Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit(E-BC-K834-M, Elabscience, China);Cell Mitochondrial Complex V (F0F1-ATPase/ATP Synthase) Activity Assay Kit (E-BC-K838-M, Elabscience, China);Cell Mitochondrial Complex III (Coenzyme Q-Cytochrome C Reductase) Activity Assay Kit(E-BC-K836-M, Elabscience, China);.

    Techniques: Membrane

    Astragaloside IV treatment of LPS-induced skeletal proteins injury through DUSP1-PHB2 interaction. (A,F-G) Detection of endoplasmic reticulum autophagy fluorescence and related gene transcription levels. Scale bar, 150 μm. (B-E) Transcription levels of endoplasmic reticulum stress-related genes. (H-M) Transcription level detection of genes related to mitochondrial unfolded protein reaction. (N-O) TOM2 fluorescence laser confocal detection (mitochondrial autophagy markers). Scale bar, 150 μm.. (P-Q) Detection of mitochondrial autophagy related genes. (R) Detection of myocardial cytoskeletal protein integrity. Scale bar, 25 μm. (S-V)Transcriptomic detection of myocardial cells before and after treatment with LPS (mitochondrial/endoplasmic reticulum related gene enrichment analysis). Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Journal: Journal of Advanced Research

    Article Title: Astragaloside IV alleviates septic myocardial injury through DUSP1-Prohibitin 2 mediated mitochondrial quality control and ER-autophagy

    doi: 10.1016/j.jare.2024.10.030

    Figure Lengend Snippet: Astragaloside IV treatment of LPS-induced skeletal proteins injury through DUSP1-PHB2 interaction. (A,F-G) Detection of endoplasmic reticulum autophagy fluorescence and related gene transcription levels. Scale bar, 150 μm. (B-E) Transcription levels of endoplasmic reticulum stress-related genes. (H-M) Transcription level detection of genes related to mitochondrial unfolded protein reaction. (N-O) TOM2 fluorescence laser confocal detection (mitochondrial autophagy markers). Scale bar, 150 μm.. (P-Q) Detection of mitochondrial autophagy related genes. (R) Detection of myocardial cytoskeletal protein integrity. Scale bar, 25 μm. (S-V)Transcriptomic detection of myocardial cells before and after treatment with LPS (mitochondrial/endoplasmic reticulum related gene enrichment analysis). Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Article Snippet: NO: Mouse MMP-9(Matrix Metalloproteinase 9) ELISA Kit E-EL-M3052, Elabscience, China);Mouse IL-10(Interleukin 10) ELISA Kit(E-EL-M0046, Elabscience, China);Mouse IL-17A(Interleukin 17A) ELISA Kit(E-EL-M0047, Elabscience, China);MDA(Malondialdehyde) ELISA Kit(E-EL-0060, Elabscience, China);A001-2-1 Superoxide Dismutase (SOD) Typing Test Kit(E-BC-K020-M, Elabscience, China);H545-1-1 Glutathione Peroxidase 4 (gpx4) Kit(E-BC-K096-S, Elabscience, China);Cell Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit(E-BC-K834-M, Elabscience, China);Cell Mitochondrial Complex V (F0F1-ATPase/ATP Synthase) Activity Assay Kit (E-BC-K838-M, Elabscience, China);Cell Mitochondrial Complex III (Coenzyme Q-Cytochrome C Reductase) Activity Assay Kit(E-BC-K836-M, Elabscience, China);.

    Techniques: Fluorescence

    Analysis of the therapeutic mechanism of Astragaloside IV on SCM myocardial cell injury through DUSP1-PHB2 interaction. (A) Volcano plot analysis of gene site differences before and after treatment with Astragaloside IV (B) Transcriptomics enrichment analysis of mitochondrial oxidative stress/oxidative phosphorylation levels (C-E) Enrichment gene analysis of pyroptosis/mitochondrial fusion/mitochondrial autophagy/endoplasmic reticulum stress/cell apoptosis/unfolded protein response difference analysis (F) Prediction of molecular docking between DUSP1 and PHB2 proteins (G) Prediction of molecular docking between Astragaloside IV and DUSP1 proteins (H) Prediction of molecular docking between Astragaloside IV and PHB2 proteins (I) The effect of B2-DUSP1 interaction mechanism on the improvement of cytoskeletal protein damage by astragaloside IV；Scale bar, 25 μm. Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Journal: Journal of Advanced Research

    Article Title: Astragaloside IV alleviates septic myocardial injury through DUSP1-Prohibitin 2 mediated mitochondrial quality control and ER-autophagy

    doi: 10.1016/j.jare.2024.10.030

    Figure Lengend Snippet: Analysis of the therapeutic mechanism of Astragaloside IV on SCM myocardial cell injury through DUSP1-PHB2 interaction. (A) Volcano plot analysis of gene site differences before and after treatment with Astragaloside IV (B) Transcriptomics enrichment analysis of mitochondrial oxidative stress/oxidative phosphorylation levels (C-E) Enrichment gene analysis of pyroptosis/mitochondrial fusion/mitochondrial autophagy/endoplasmic reticulum stress/cell apoptosis/unfolded protein response difference analysis (F) Prediction of molecular docking between DUSP1 and PHB2 proteins (G) Prediction of molecular docking between Astragaloside IV and DUSP1 proteins (H) Prediction of molecular docking between Astragaloside IV and PHB2 proteins (I) The effect of B2-DUSP1 interaction mechanism on the improvement of cytoskeletal protein damage by astragaloside IV；Scale bar, 25 μm. Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Article Snippet: NO: Mouse MMP-9(Matrix Metalloproteinase 9) ELISA Kit E-EL-M3052, Elabscience, China);Mouse IL-10(Interleukin 10) ELISA Kit(E-EL-M0046, Elabscience, China);Mouse IL-17A(Interleukin 17A) ELISA Kit(E-EL-M0047, Elabscience, China);MDA(Malondialdehyde) ELISA Kit(E-EL-0060, Elabscience, China);A001-2-1 Superoxide Dismutase (SOD) Typing Test Kit(E-BC-K020-M, Elabscience, China);H545-1-1 Glutathione Peroxidase 4 (gpx4) Kit(E-BC-K096-S, Elabscience, China);Cell Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit(E-BC-K834-M, Elabscience, China);Cell Mitochondrial Complex V (F0F1-ATPase/ATP Synthase) Activity Assay Kit (E-BC-K838-M, Elabscience, China);Cell Mitochondrial Complex III (Coenzyme Q-Cytochrome C Reductase) Activity Assay Kit(E-BC-K836-M, Elabscience, China);.

    Techniques: Phospho-proteomics

    Astragaloside IV treatment of LPS-induced MQS dysfunction in cardiomyocytes through DUSP1-PHB2 interaction. (A) Fluorescence expression of TOM20 (laser confocal detection) Scale bar,150 μm. (B, G-I,U) Expression levels of mitochondrial biosynthesis related proteins (C-F,U) Detection of mitochondrial autophagy related proteins and gene expression levels (J-N) Detection of mitochondrial dynamics (fusion/fission) related gene transcription levels and mitochondrial structure (Mito tracker) Scale bar, 25 μm. (O-P) Detection of fluorescence expression and transcription levels of DUSP1 Scale bar, 100 μm; (Q-T) Detection of mitochondrial energy metabolism；Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Journal: Journal of Advanced Research

    Article Title: Astragaloside IV alleviates septic myocardial injury through DUSP1-Prohibitin 2 mediated mitochondrial quality control and ER-autophagy

    doi: 10.1016/j.jare.2024.10.030

    Figure Lengend Snippet: Astragaloside IV treatment of LPS-induced MQS dysfunction in cardiomyocytes through DUSP1-PHB2 interaction. (A) Fluorescence expression of TOM20 (laser confocal detection) Scale bar,150 μm. (B, G-I,U) Expression levels of mitochondrial biosynthesis related proteins (C-F,U) Detection of mitochondrial autophagy related proteins and gene expression levels (J-N) Detection of mitochondrial dynamics (fusion/fission) related gene transcription levels and mitochondrial structure (Mito tracker) Scale bar, 25 μm. (O-P) Detection of fluorescence expression and transcription levels of DUSP1 Scale bar, 100 μm; (Q-T) Detection of mitochondrial energy metabolism；Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Article Snippet: NO: Mouse MMP-9(Matrix Metalloproteinase 9) ELISA Kit E-EL-M3052, Elabscience, China);Mouse IL-10(Interleukin 10) ELISA Kit(E-EL-M0046, Elabscience, China);Mouse IL-17A(Interleukin 17A) ELISA Kit(E-EL-M0047, Elabscience, China);MDA(Malondialdehyde) ELISA Kit(E-EL-0060, Elabscience, China);A001-2-1 Superoxide Dismutase (SOD) Typing Test Kit(E-BC-K020-M, Elabscience, China);H545-1-1 Glutathione Peroxidase 4 (gpx4) Kit(E-BC-K096-S, Elabscience, China);Cell Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit(E-BC-K834-M, Elabscience, China);Cell Mitochondrial Complex V (F0F1-ATPase/ATP Synthase) Activity Assay Kit (E-BC-K838-M, Elabscience, China);Cell Mitochondrial Complex III (Coenzyme Q-Cytochrome C Reductase) Activity Assay Kit(E-BC-K836-M, Elabscience, China);.

    Techniques: Fluorescence, Expressing, Gene Expression

    Astragaloside IV treatment of LPS-induced ER-autophagy in cardiomyocytes through DUSP1-PHB2 interaction. (A-B) Laser confocal detection of the endoplasmic reticulum. Scale bar, 25 μm (C-E) Detection of Complex I/III/V activity; (F) Detection of VDAC1 transcription level; (G) Detection of mPTP open level; (H-M) Detection of transcription levels of genes related to the mitochondrial unfolded protein response; (N) Detection of expression levels of pan-apoptosis related regulatory proteins; (O) Detection of endoplasmic reticulum autophagy expression level; Scale bar, 125 μm (P-S) Detection of transcription levels of pan-apoptosis related genes.Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Journal: Journal of Advanced Research

    Article Title: Astragaloside IV alleviates septic myocardial injury through DUSP1-Prohibitin 2 mediated mitochondrial quality control and ER-autophagy

    doi: 10.1016/j.jare.2024.10.030

    Figure Lengend Snippet: Astragaloside IV treatment of LPS-induced ER-autophagy in cardiomyocytes through DUSP1-PHB2 interaction. (A-B) Laser confocal detection of the endoplasmic reticulum. Scale bar, 25 μm (C-E) Detection of Complex I/III/V activity; (F) Detection of VDAC1 transcription level; (G) Detection of mPTP open level; (H-M) Detection of transcription levels of genes related to the mitochondrial unfolded protein response; (N) Detection of expression levels of pan-apoptosis related regulatory proteins; (O) Detection of endoplasmic reticulum autophagy expression level; Scale bar, 125 μm (P-S) Detection of transcription levels of pan-apoptosis related genes.Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Article Snippet: NO: Mouse MMP-9(Matrix Metalloproteinase 9) ELISA Kit E-EL-M3052, Elabscience, China);Mouse IL-10(Interleukin 10) ELISA Kit(E-EL-M0046, Elabscience, China);Mouse IL-17A(Interleukin 17A) ELISA Kit(E-EL-M0047, Elabscience, China);MDA(Malondialdehyde) ELISA Kit(E-EL-0060, Elabscience, China);A001-2-1 Superoxide Dismutase (SOD) Typing Test Kit(E-BC-K020-M, Elabscience, China);H545-1-1 Glutathione Peroxidase 4 (gpx4) Kit(E-BC-K096-S, Elabscience, China);Cell Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit(E-BC-K834-M, Elabscience, China);Cell Mitochondrial Complex V (F0F1-ATPase/ATP Synthase) Activity Assay Kit (E-BC-K838-M, Elabscience, China);Cell Mitochondrial Complex III (Coenzyme Q-Cytochrome C Reductase) Activity Assay Kit(E-BC-K836-M, Elabscience, China);.

    Techniques: Activity Assay, Expressing

    Astragaloside IV improved myocardial function through DUSP1-PHB2 in the treatment of septic myocarditis injury. (A) Immunofluorescence staining showed the expression of Tnt and Gr-1 in cardiac tissue (DAPI was used as internal reference staining). Scale bar, 150 μm (B-D) Echocardiography was used to analyze the cardiac function of mice. The parameters analyzed included left ventricular ejection fraction (LVEF%),FS%, and LVDd. (E-G) Serum levels of myocardial injury markers, including creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), and troponin (TnT), were detected. (H-J) Analysis of apoptosis-related indicators, including the expression levels of caspase-1, caspase-3, and caspase-9. (K-P) Detection of transcription levels for genes associated with the mitochondrial unfolded protein response. (T-U) Detection of expression levels for proteins related to mitochondrial dynamics and endoplasmic reticulum stress.. Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Journal: Journal of Advanced Research

    Article Title: Astragaloside IV alleviates septic myocardial injury through DUSP1-Prohibitin 2 mediated mitochondrial quality control and ER-autophagy

    doi: 10.1016/j.jare.2024.10.030

    Figure Lengend Snippet: Astragaloside IV improved myocardial function through DUSP1-PHB2 in the treatment of septic myocarditis injury. (A) Immunofluorescence staining showed the expression of Tnt and Gr-1 in cardiac tissue (DAPI was used as internal reference staining). Scale bar, 150 μm (B-D) Echocardiography was used to analyze the cardiac function of mice. The parameters analyzed included left ventricular ejection fraction (LVEF%),FS%, and LVDd. (E-G) Serum levels of myocardial injury markers, including creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), and troponin (TnT), were detected. (H-J) Analysis of apoptosis-related indicators, including the expression levels of caspase-1, caspase-3, and caspase-9. (K-P) Detection of transcription levels for genes associated with the mitochondrial unfolded protein response. (T-U) Detection of expression levels for proteins related to mitochondrial dynamics and endoplasmic reticulum stress.. Data are shown as mean ± SEM. Ten animals were used in each group and the dotes in each panel represent the average data of three replicates in each animal. *p < 0.05.

    Article Snippet: NO: Mouse MMP-9(Matrix Metalloproteinase 9) ELISA Kit E-EL-M3052, Elabscience, China);Mouse IL-10(Interleukin 10) ELISA Kit(E-EL-M0046, Elabscience, China);Mouse IL-17A(Interleukin 17A) ELISA Kit(E-EL-M0047, Elabscience, China);MDA(Malondialdehyde) ELISA Kit(E-EL-0060, Elabscience, China);A001-2-1 Superoxide Dismutase (SOD) Typing Test Kit(E-BC-K020-M, Elabscience, China);H545-1-1 Glutathione Peroxidase 4 (gpx4) Kit(E-BC-K096-S, Elabscience, China);Cell Mitochondrial Complex I (NADH-CoQ Reductase) Activity Assay Kit(E-BC-K834-M, Elabscience, China);Cell Mitochondrial Complex V (F0F1-ATPase/ATP Synthase) Activity Assay Kit (E-BC-K838-M, Elabscience, China);Cell Mitochondrial Complex III (Coenzyme Q-Cytochrome C Reductase) Activity Assay Kit(E-BC-K836-M, Elabscience, China);.

    Techniques: Immunofluorescence, Staining, Expressing